| Autor: |
Xu An, Xiangning Li, Corey Elowsky, Laura Korobkova, Lei Gao, Giorgio A. Ascoli, Judith Mizrachi, William Galbavy, Jesse Gillis, Byung Kook Lim, Hanchuan Peng, Katherine Matho, Jason Palmer, Liya Ding, Uree Chon, Michael Hawrylycz, Florence D’Orazi, Kathleen Kelly, James C. Gee, Anastasiia Bludova, Peter A. Groblewski, Xu Li, Z. Josh Huang, Wayne Wakemen, Yun Wang, Stephanie Mok, Xiuli Kuang, X. William Yang, Houri Hintiryan, Michael S. Bienkowski, Karla E. Hirokawa, Lydia Ng, Peng Xie, Diek W. Wheeler, Xiaoyin Chen, Jin Yuan, Julie A. Harris, Yaoyao Li, Ramesh Palaniswamy, Yongsoo Kim, Li I. Zhang, Qingming Luo, Darrick Lo, Samik Bannerjee, Huizhong Tao, Ian Bowman, Meng Kuan Lin, Yu-Chi Sun, Quanxin Wang, Elise Shen, Lijuan Liu, Joel D. Hahn, Joshua T. Hatfield, Anan Li, Maitham Naeemi, Hui Gong, Xiaoli Qi, Partha P. Mitra, Hideki Kondo, Philip R. Nicovich, Pavel Osten, Hongkui Zeng, Muye Zhu, Sarojini M. Attili, Brian Zingg, Anthony M. Zador, Stephan Fischer, Bing-Xing Huo, Shenqin Yao, Nicholas N. Foster, Philip Lesnar, Rodrigo Muñoz-Castañeda, Ali Cetin, Young Gyun Park, Arun Narasimhan, Kwanghun Chuang, Lin Gou, Hong-Wei Dong, Yimin Wang, Chris Sin Park, Rhonda Drewes, Jason Huang |
| Rok vydání: |
2020 |
| Předmět: |
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| Popis: |
An essential step toward understanding brain function is to establish a cellular-resolution structural framework upon which multi-scale and multi-modal information spanning molecules, cells, circuits and systems can be integrated and interpreted. Here, through a collaborative effort from the Brain Initiative Cell Census Network (BICCN), we derive a comprehensive cell type-based description of one brain structure - the primary motor cortex upper limb area (MOp-ul) of the mouse. Applying state-of-the-art labeling, imaging, computational, and neuroinformatics tools, we delineated the MOp-ul within the Mouse Brain 3D Common Coordinate Framework (CCF). We defined over two dozen MOp-ul projection neuron (PN) types by their anterograde targets; the spatial distribution of their somata defines 11 cortical sublayers, a significant refinement of the classic notion of cortical laminar organization. We further combine multiple complementary tracing methods (classic tract tracing, cell type-based anterograde, retrograde, and transsynaptic viral tracing, high-throughput BARseq, and complete single cell reconstruction) to systematically chart cell type-based MOp input-output streams. As PNs link distant brain regions at synapses as well as host cellular gene expression, our construction of a PN type resolution MOp-ul wiring diagram will facilitate an integrated analysis of motor control circuitry across the molecular, cellular, and systems levels. This work further provides a roadmap towards a cellular resolution description of mammalian brain architecture. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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