Autor: |
U. Persson, I. Dahlqvist, C.-B. Laurell |
Rok vydání: |
1983 |
Předmět: |
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Zdroj: |
Journal of Chromatography B: Biomedical Sciences and Applications. 278:53-61 |
ISSN: |
0378-4347 |
Popis: |
A method has been developed for the rapid isolation of α1 -antitrypsin and other thiol proteins from plasma by an automated chromatography system. The thiol-proteins are initially bound to matrix-linked activated thiol-compounds by an SHSS interchange reaction. The mixed disulphides are then reduced in two steps and subfractionated by passage through Blue-Sepharose and AH-Sepharose columns. The rate of the interchange reactions varies with the microenvironment of the reacting thiols. α1-Antitrypsin is recovered with 95% purity in 60% yield within two days from 1 l of plasma. |
Databáze: |
OpenAIRE |
Externí odkaz: |
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