The use of thiol-disulphide exchange chromatography for the automated isolation of oil -antitrypsin and other plasma proteins with reactive thiol groups

Autor:	U. Persson, I. Dahlqvist, C.-B. Laurell
Rok vydání:	1983
Předmět:	chemistry.chemical_classification α1 antitrypsin Chromatography Enzyme chemistry Affinity chromatography Yield (chemistry) Blood plasma Thiol Mixed disulphides General Chemistry Blood proteins
Zdroj:	Journal of Chromatography B: Biomedical Sciences and Applications. 278:53-61
ISSN:	0378-4347
Popis:	A method has been developed for the rapid isolation of α1 -antitrypsin and other thiol proteins from plasma by an automated chromatography system. The thiol-proteins are initially bound to matrix-linked activated thiol-compounds by an SHSS interchange reaction. The mixed disulphides are then reduced in two steps and subfractionated by passage through Blue-Sepharose and AH-Sepharose columns. The rate of the interchange reactions varies with the microenvironment of the reacting thiols. α1-Antitrypsin is recovered with 95% purity in 60% yield within two days from 1 l of plasma.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::f61b70a0fa4ea1ad782017d52fc9bb45 https://doi.org/10.1016/s0378-4347(00)84755-6 Zobrazit plný text záznamu