Convenient enzymatic preparation of conjugated linoleic acid alkyl esters with C6–C22 alcohols
| Autor: | László Poppe, Katalin Recseg, Csilla Dianoczki, Katalin Kővári |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
chemistry.chemical_classification
Wax biology Process Chemistry and Technology Conjugated linoleic acid Triacylglycerol lipase food and beverages Bioengineering Alcohol biology.organism_classification Biochemistry Catalysis chemistry.chemical_compound Enzyme chemistry visual_art biology.protein visual_art.visual_art_medium Organic chemistry lipids (amino acids peptides and proteins) Candida antarctica Lipase Alkyl |
| Zdroj: | Journal of Molecular Catalysis B: Enzymatic. 45:45-49 |
| ISSN: | 1381-1177 |
| Popis: | A fast and effective enzymatic process was developed for preparing CLA C6–C22 alkyl esters using immobilized Candida antarctica lipase B (Novozym 435). The reaction parameters (temperature, amount of starting materials and enzyme, effect of water removal during the reaction) were optimized. The re-use study showed that the enzyme can be used at least ten times without significant loss in its activity. Reactions between equimolar amounts of starting materials with 8.9% Novozym 435 (w/w CLA ) at 65 °C in vacuum (20 mbar) for 1.5 h resulted in almost quantitatively (>95%) wax esters needing no further treatment. At room temperature, CLA esters with long chain (≥18 °C) saturated alcohols are solids. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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