A two-dimensional protein map ofCaenorhabditis elegans
| Autor: | Claes Wahlestedt, Hanno Langen, Sabine P. Schrimpf, Ana Vaz Gomes |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Gel electrophoresis
education.field_of_study Two-dimensional gel electrophoresis Chromatography biology Clinical Biochemistry Population Trypsin biology.organism_classification Mass spectrometry Biochemistry Analytical Chemistry Peptide mass fingerprinting medicine Immobilized pH gradient education Caenorhabditis elegans medicine.drug |
| Zdroj: | ELECTROPHORESIS. 22:1224-1232 |
| ISSN: | 1522-2683 0173-0835 |
| DOI: | 10.1002/1522-2683()22:6<1224::aid-elps1224>3.0.co;2-i |
| Popis: | A protein map of Caenorhabditis elegans was constructed by using two-dimensional gel electrophoresis followed by peptide mass fingerprinting. A whole worm extract of a mixed population was separated on immobilized pH gradient strips 4-7 L, 3-10 NL, 6-11 L and 12% sodium dodecyl sulfate-polyacrylamide gel eletrophoresis (SDS-PAGE) gels. Gels were stained with colloidal Coomassie blue and 286 spots representing 152 proteins were subsequently identified by matrix-assisted laser desorption/ionization-mass spectrometry after in-gel digestion with trypsin. Most of the identified proteins with known cellular function were enzymes related to carbohydrate and lipid metabolism, or structural proteins with subcellular locations in the cytoplasm, mitochondria or cytoskeleton. |
| Databáze: | OpenAIRE |
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