Isolation and characterization of polymorphic microsatellites in diploid strawberry (Fragaria vesca L.) for mapping, diversity studies and clone identification
Autor: | F. Wilson, A. M. Hadonou, C. M. James, K. R. Tobutt |
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Rok vydání: | 2003 |
Předmět: | |
Zdroj: | Molecular Ecology Notes. 3:171-173 |
ISSN: | 1471-8286 1471-8278 |
DOI: | 10.1046/j.1471-8286.2003.00365.x |
Popis: | This study reports the development and characterization of 10 polymorphic microsatellite primer pairs in wild strawberry (Fragaria vesca). The primers were designed from a genomic library enriched for di-, tri- and tetranucleotide repeats from F. vesca‘Reugen’. They showed single locus polymorphism in a set of nine F. vesca accessions; two to six alleles were detected per locus. The level of polymorphism in F. vesca was surprisingly low, although three pairs of primers were sufficient to distinguish between most accessions. |
Databáze: | OpenAIRE |
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