Method development and validation of Montelukast sodium in bulk and tablet formulation by HPLC
| Autor: | Dipti G. Phadtare, Govind K. Patil, Amol R. Pawar, Rajashri R. Kulkarni |
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| Rok vydání: | 2016 |
| Předmět: |
Detection limit
Chromatography 010401 analytical chemistry 02 engineering and technology 021001 nanoscience & nanotechnology 01 natural sciences High-performance liquid chromatography Method development Quantitative determination Dosage form 0104 chemical sciences Linear relationship Montelukast Sodium Linear regression 0210 nano-technology Mathematics Biomedical engineering |
| Zdroj: | Asian Journal of Research in Chemistry. 9:339 |
| ISSN: | 0974-4150 0974-4169 |
| DOI: | 10.5958/0974-4150.2016.00051.1 |
| Popis: | A high performance liquid chromatography method has been developed and validated method for the simultaneous estimation of Montelukast sodium in bulk and tablet dosage form. The method was carried out using Princeton SPHER ULTIMA C18 100A 5μ 250*4.6 mm using OPA: Methanol (10: 90 v/v) as mobile phase at flow rate 1ml/min. HPLC separation was carried out at 284 nm. The linear regression analysis data for the calibration plots showed good linear relationship with r2 = 0.9982 and 0.9979 for Montelukast sodium in bulk and tablet dosage form respectively in the concentration range of 1–6 μl/ml for Montelukast sodium in bulk and tablet dosage form. The method was validated for precision, robustness, specificity and accuracy. The limit of detection and quantitation were 0.0011 and 0.0033 μg/ml. the proposed HPLC method can be applied for identification and quantitative determination of Montelukast sodium in bulk and tablet dosage form. |
| Databáze: | OpenAIRE |
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