Characterization of the bottom sediments contaminated with polychlorinated biphenyls: Evaluation of ecotoxicity and biodegradability
| Autor: | Lívia Tóthová, Katarína Šilhárová, Katarína Dercová, Mária Mikulášová, Hana Dudášová, Jana Šeligová, Pavel Hucko |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
education.field_of_study
biology Chemistry Population food and beverages Soil classification Microbial consortium biology.organism_classification Microbiology Soil contamination Pseudomonas stutzeri Ames test Biomaterials Biostimulation Environmental chemistry Ecotoxicity education Waste Management and Disposal |
| Zdroj: | International Biodeterioration & Biodegradation. 63:440-449 |
| ISSN: | 0964-8305 |
| DOI: | 10.1016/j.ibiod.2008.12.005 |
| Popis: | At the locality of the former producer of PCBs Chemko Stražske in East Slovakia, a large amount of PCBs (the commercial mixture DELOR 103, an equivalent of AROCLOR 1242) is still persisting in sediments and negatively influences health of the population. The objective of this work was to provide a study of ecotoxicity and genotoxicity of PCBs in contaminated sediments. Toxicity of the PCB-contaminated sediments sampled from Zemplinska sirava and Stražsky canal (surroundings of the former producer of PCBs) was determined applying a standard aquatic plant toxicity test using Lemna minor . The endpoints for the test were frond numbers and frond areas. The sediment sampled from Zemplinska sirava was more toxic to L. minor than the one sampled from Stražsky canal. The results on genotoxicity showed that both sediments were not mutagenic toward the standard strains of the Ames test, Salmonella typhimurium TA98 and TA100. This work deals also with biodegradation of PCBs in two samples of the above mentioned contaminated sediments: a) in the natural sediments by autochthonous microbial consortium and b) in the bioaugmented sediments inoculated by allochthonous bacterial strains, two bacterial isolates from long-term PCB-contaminated soil Pseudomonas stutzeri and Alcaligenes xylosoxidans . Both approaches were applied under the biostimulation conditions, with addition of glucose or biphenyl as co-substrates, as well. The highest PCB degradation was observed in the bioaugmented sediment inoculated with bacterial strain P. stutzeri . Addition of biphenyl, as the co-substrate and the inducer, positively affected degradation of PCBs. The bphA1 gene, encoding enzyme biphenyldioxygenase, responsible for the start of PCB degradation, was identified in genome of P. stutzeri , a potential PCB-degrader isolated from long-term PCB-contaminated soil, but not in genome of A. xylosoxidans . |
| Databáze: | OpenAIRE |
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