Isolation and Identification of GABA-producing Microorganism from Chungkookjang

Autor:	Dae Youn Hwang, Ro-Ui Kim, Dong-Seob Kim, Eun-Ah Kim, So-Yon Mann, Hong-Joo Son, Ga-Young Lee
Rok vydání:	2013
Předmět:	Protease Strain (chemistry) Stationary phase Microorganism medicine.medical_treatment medicine Bacillus subtilis Biology 16S ribosomal RNA Isolation (microbiology) biology.organism_classification Incubation Microbiology
Zdroj:	Journal of Life Science. 23:102-109
ISSN:	1225-9918
DOI:	10.5352/jls.2013.23.1.102
Popis:	To isolate GABA-producing microorganisms, 1,500 strains were isolated from different Chungkookjang samples and screened. From these strains, 20 were selected for further analyses based on a protease and slime-producing activity test. The MC 31 strain showed the highest GABA concentration in Chungkookjang and was used in this study. MC 31 was identified as Bacillus subtilis by an API 50CHB kit and 16S rDNA sequences analysis and named as B. subtilis MC 31. B. subtilis MC 31 showed exponential growth up to 12 hours at in LB broth, and it reached a stationary phase after 24 to 36 hours of incubation. B. subtilis MC 31 showed maximum GABA content at 72 hours after incubation at .
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::f5f7b744de71db4bf0d5ed66b368fa4b https://doi.org/10.5352/jls.2013.23.1.102 Zobrazit plný text záznamu