Versatile Application of Fluorescent Quantum Dot Labels in Super-resolution Fluorescence Microscopy
| Autor: | Juntao Gao, Hening Wang, Yujia Liu, Karl Zhanghao, Kebin Shi, Peng Xi, Fan Wang, Xu Zhang, Dayong Jin, Xusan Yang |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Materials science Super-resolution microscopy Nanotechnology 02 engineering and technology Broadband excitation 021001 nanoscience & nanotechnology Fluorescence Superresolution Atomic and Molecular Physics and Optics Electronic Optical and Magnetic Materials 03 medical and health sciences 030104 developmental biology Quantum dot Microscopy Fluorescence microscope Electrical and Electronic Engineering 0210 nano-technology Biotechnology |
| Zdroj: | ACS Photonics. 3:1611-1618 |
| ISSN: | 2330-4022 |
| DOI: | 10.1021/acsphotonics.6b00178 |
| Popis: | Quantum dots (QDs) are well known as bright and photostable inorganic fluorescent probes for microscopy imaging, with many attractive features superior to those found in organic dyes. However, their broadband excitation spectrum and emission blinking property have limited the applicability of QDs in modern super-resolution microscopy techniques. In this work, we systematically investigate practical approaches to overcoming these drawbacks and provide examples of their use across many commercially available super-resolution microscopy systems now accessible to biologists, with examples across the major super-resolution techniques. This work further maps out how QDs can be further engineered to facilitate their applications in the respective super-resolution microscopy techniques. |
| Databáze: | OpenAIRE |
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