P16ink4a Immunoexpression Profile in HPV-Oral Lesions from HIV-Infected Patients
| Autor: | García-Carrancá Alej, Irigoyen-Camacho Esther, Anaya-Saavedra Gabriela, Ramírez-Amador Velia, Ortíz-Sánchez Elizabeth, Rosales-Terrazas Estrella, Guido-Jiménez Miriam |
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| Rok vydání: | 2015 |
| Předmět: |
medicine.medical_specialty
Pathology Surrogate endpoint business.industry Immunology Human immunodeficiency virus (HIV) virus diseases Cancer Dermatology Hyperplasia medicine.disease medicine.disease_cause Omics female genital diseases and pregnancy complications Infectious Diseases Acquired immunodeficiency syndrome (AIDS) Virology Immunohistochemistry Medicine Histopathology business neoplasms |
| Zdroj: | Journal of AIDS & Clinical Research. |
| ISSN: | 2155-6113 |
| DOI: | 10.4172/2155-6113.1000411 |
| Popis: | Background: In HIV-patients, a proportion of benign HPV-associated oral lesions (HPV-OLs) contain high risk- HPV (HR-HPV) sequences. Recent studies demonstrated a p16INK4a overexpression in HPV-induced cancer and dysplastic lesions through pRB degradation by the E7-HPV oncoprotein; so, it has been considered a surrogate marker of HR-HPV oncogenic activity. Objective: To establish the p16INK4a expression in HPV-OL from HIV-infected patients. Materials and method: A cross-sectional study was conducted in three HIV/AIDS referral centers in Mexico City. We performed histopathological diagnosis, HPV-DNA amplification, direct sequencing, and p16INK4a immunohistochemical staining in HPV-OLs. The U-Mann-Whitney, X2, and Fisher’s exact tests were used to determine the association between variables. Result: In a total of 849 adult HIV-individuals examined, we found 29 (3.4%) patients with HPV-OLs, being multifocal epithelial hyperplasia (51.7%) the most common. Low-risk-HPV (LR-HPV) types were identified in 82.7% and HR-HPV in 10.3%. HPV-OLs exhibited a moderate/strong but only nuclear p16INK4a immunoexpression; no correlation between p16INK4a expression and HPV type were found. Conclusion: In HPV-OLs from HIV-infected patients, the comparable p16INK4a immunoexpression, independently of the specific HPV-type, as well as the absence of cytoplasmic staining, may suggest a lack of HR-HPV activity. Longitudinal studies based on oncogenic viral gene expression are warranted. |
| Databáze: | OpenAIRE |
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