Rotavirus A associated pathology of intestine and mesenteric lymph nodes and occurrence in bovine calves of Gwalior and Bareilly regions
| Autor: | Parmanand Kumar, K.P. Singh, Gayatri Kashyap, Y. S. Malik, Rakesh Agrawal, Rahul Singh, R. P. Singh, Deepanshu Gupta |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Pathology General Veterinary 0402 animal and dairy science 04 agricultural and veterinary sciences Biology medicine.disease_cause 040201 dairy & animal science Peripheral blood mononuclear cell Small intestine 03 medical and health sciences 030104 developmental biology medicine.anatomical_structure Real-time polymerase chain reaction Antigen Rotavirus medicine Mesenteric lymph nodes Animal Science and Zoology Histopathology Feces |
| Zdroj: | Indian Journal of Animal Research. |
| ISSN: | 0976-0555 0367-6722 |
| Popis: | To understand the pathology of natural cases of rotavirus (RVA) in bovine calves, a total of 40 cases below 6 months died due to diarrhoea were studied, out of which 7 cases (17.5%) turned positive for RVA by RT-PCR. Histopathology of small intestine showed loss of villous enterocytes, blunting and fusion of villi, elongation of crypts and mononuclear cells infiltration in the lamina-propria. The mesenteric lymph nodes were severely depleted of lymphocytes. These changes were corroborated with presence of RVA antigen in sections by dFAT and nucleic acid by RT-PCR. The fluorescent signals were more in mesenteric lymph nodes than in intestine. Besides, 115 rectal fecal samples were also collected from calves for RVA detection by RT-PCR using VP6 gene specific sets of primers. Dead carcasses of calves (n= 40) belonged to organized dairy farm of Bareilly, while rectal fecal samples belonged to both organized (n= 38) and unorganized farms (n= 77) of Bareilly and Gwalior. The overall occurrence of RVA was 19.3% (30/155), comprising 5/37 cases (13.5%) from Gwalior (MP) and 25/118 cases (21.1%) from Bareilly (UP). These findings suggest the infection of RVA widely prevalent in calves and have potential to escape from the intestinal site to mesenteric lymph nodes. |
| Databáze: | OpenAIRE |
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