Positive contrast from cells labeled with iron oxide nanoparticles: Quantitation of imaging data
| Autor: | Geetha Mohan, Jinjin Zhang, Djaudat S. Idiyatullin, Nancy E Lane, Sergey Magnitsky, Sharmila Majumdar, Michael Garwood |
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| Rok vydání: | 2017 |
| Předmět: |
medicine.diagnostic_test
Mesenchymal stem cell Iron oxide Magnetic resonance imaging Pulse sequence Imaging phantom 030218 nuclear medicine & medical imaging 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Nuclear magnetic resonance chemistry Flip angle medicine Radiology Nuclear Medicine and imaging Viability assay 030217 neurology & neurosurgery Iron oxide nanoparticles |
| Zdroj: | Magnetic Resonance in Medicine. 78:1900-1910 |
| ISSN: | 0740-3194 |
| DOI: | 10.1002/mrm.26585 |
| Popis: | Author(s): Magnitsky, Sergey; Zhang, Jinjin; Idiyatullin, Djaudat; Mohan, Geetha; Garwood, Michael; Lane, Nancy E; Majumdar, Sharmila | Abstract: PurposeConventional T2 -weighted MRI produces a hypointense signal from iron-labeled cells, which renders quantification unfeasible. We tested a SWeep Imaging with Fourier Transformation (SWIFT) MRI pulse sequence to generate a quantifiable hyperintense signal from iron-labeled cells.MethodsMesenchymal stem cells (MSCs) were labeled with different concentrations of iron oxide particles and examined for cell viability, proliferation, and differentiation. The SWIFT sequence was optimized to detect and quantify the amount of iron in the muscle tissue after injection of iron oxide solution and iron-labeled MSCs.ResultsThe incubation of MSCs with iron oxide and low concentration of poly-L-lysine mixture resulted in an internalization of up to 22 pg of iron per cell with no adverse effect on MSCs. Phantom experiments showed a dependence of SWIFT signal intensity on the excitation flip angle. The hyperintense signal from iron-labeled cells or solutions was detected, and an amount of the iron oxide in the tissue was quantified with the variable flip angle method.ConclusionsThe SWIFT sequence can produce a quantifiable hyperintense MRI signal from iron-labeled cells. The graft of 18 x 106 cells was detectable for 19 days after injection and the amount of iron was quantifiable. The proposed protocol simplifies the detection and provides a means to quantify cell numbers. Magn Reson Med 78:1900-1910, 2017. © 2017 International Society for Magnetic Resonance in Medicine. |
| Databáze: | OpenAIRE |
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