| Autor: |
P. Lintu, Markku Viander, Erkki O. Terho, J. Kosonen, Jean Bousquet, Jérôme Pène, Kirsti Kalimo, J. Savolainen |
| Rok vydání: |
1999 |
| Předmět: |
|
| Zdroj: |
Clinical & Experimental Allergy. 29:824-831 |
| ISSN: |
0954-7894 |
| Popis: |
Background The cytokine observed most often in atopic dermatitis (AD) is IL-4, but a role for IL-5 and IFN-γ in the late and delayed phase reactions has been suggested. In AD with head, neck and shoulder distribution, hypersensitivity to saprophytic yeasts is an important pathogenetic factor. The yeast allergens include both the mannan polysaccharides and the proteins. Mannans are major cross-reacting allergens likely to be involved in the pathogenesis of AD. Objective To characterize the humoral, lymphoproliferative and cytokine (IL-2, 4, 5 and IFN-γ) responses of peripheral blood mononuclear cells (PBMCs) induced by Candida albicans mannan and protein antigens in AD. Methods Fifteen AD patients and seven healthy controls were included. Ficoll-isolated PBMCs were stimulated by PHA and laboratory-generated mannan and protein extracts of C. albicans. Lymphocyte proliferation was measured and cytokine production was studied by ELISA. The antigen-specific IgG and IgE antibodies were analysed by ELISA and nitrocellulose RAST. Results In AD mannan (P |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
