Undifferentiated Neuroblastoma Cells Are More Sensitive to Photogenerated Oxidative Stress Than Differentiated Cells

Autor:	Huang-Yo Chen, Yi-Ren Hong, Jyh-Jye Wang, Jing-Huei Perng, Chu-I Lee
Rok vydání:	2015
Předmět:	MAPK/ERK pathway medicine.medical_treatment Cellular differentiation Photodynamic therapy Cell Biology Biology medicine.disease_cause medicine.disease Biochemistry Cell biology Apoptosis Neuroblastoma medicine Undifferentiated Neuroblastoma Molecular Biology Protein kinase B Oxidative stress
Zdroj:	Journal of Cellular Biochemistry. 116:2074-2085
ISSN:	0730-2312
Popis:	Neuroblastoma is one of the most aggressive cancers and has a complex form of differentiation. We hypothesized that advanced cellular differentiation may alter the susceptibility of neuroblastoma to photodynamic treatment (PDT) and confer selective survival advantage. We demonstrated that hematoporphyrin uptake by undifferentiated SH-SY5Y cells was lower than that of differentiated counterparts, yet the former were more susceptible to PDT-induced oxidative stress killing. Photogenerated reactive oxygen species (ROS) in undifferentiated cells efficiently stimulated cell cycle arrest at G2/M phase, mitochondrial apoptotic pathway activation, the sustained phosphorylation of Akt/GSK-3β and ERK. Differentiated cells with more resistance to PDT exhibited a ROS-independent and a prolonged activation of ERK. Both SH-SY5Y cells exposed to PDT exhibited ROS-independent p38 and JNK activation. These results may have important implications for neuroblastoma patients undergoing photodynamic therapy.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::f5bb2417dddf9d3b0d71d6cdcbeda875 https://doi.org/10.1002/jcb.25165 Zobrazit plný text záznamu Plný text