Study of biological properties of field isolates of cattle minor infections agents on homological cell cultures

Autor:	V. P. Gorbatenko, N. Rudova, O. M. Kornieikov, O. Yu. Lymanska, I. O. Kulyk, O. V. Kuznetsova, O. V. Dunaeva, M. Yu. Stegniy, S. K. Gorbatenko, O. S. Solodiankin
Rok vydání:	2020
Předmět:	0301 basic medicine 0403 veterinary science 03 medical and health sciences 030104 developmental biology Field (physics) 040301 veterinary sciences Cell culture Biological property 04 agricultural and veterinary sciences Biology Microbiology
Zdroj:	Journal for Veterinary Medicine, Biotechnology and Biosafety. 6:16-19
ISSN:	2411-0388 2411-3174
DOI:	10.36016/jvmbbs-2020-6-2-3
Popis:	Biological properties of field isolates of bovine immunodeficiency virus and bovine foamy virus on homological cell cultures (fetal bovine lung and bovine coronary artery endothelial cells) were investigated. Pathogens of bovine slow infections, namely bovine immunodeficiency virus and bovine foamy virus, are able to integrate into cell cultures of homologous to cattle type, which is confirmed by the results of PCR. There has been determined the presence of genetic material of pathogens of bovine immunodeficiency (BIV) and spumavirus infection (BFV) in the cultivation of lymphocytes of field isolates in the culture of bovine coronary artery endothelial cells (BCAEC) at the level of 5th passage, and in the cell culture of fetal bovine lung (FBL) — at the level of 10th passage. In the process of integration of pathogens of immunodeficiency and spumavirus infection of cattle in continuous cell cultures FBL and BCAEC, morphological changes in the state of the monolayer by the principle of syncytiation and vacuolation are observed
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::f59f1b00de37de3c3c6dc3b32220d43d https://doi.org/10.36016/jvmbbs-2020-6-2-3 Zobrazit plný text záznamu