| Autor: |
Monica A. Spiteri, A. H. Mansur, Anthony A. Fryer, Michael Hepple, Richard C. Strange |
| Rok vydání: |
2002 |
| Předmět: |
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| Zdroj: |
Clinical & Experimental Allergy. 32:994-999 |
| ISSN: |
0954-7894 |
| DOI: |
10.1046/j.1365-2222.2002.01426.x |
| Popis: |
BACKGROUND Previously, an association has been reported between an increased risk of asthma and a polymorphism in the Clara cell secretory protein (CC16) gene [namely, an adenine to guanine substitution in the CC16 gene at position 38 (A38G) downstream from the transcription initiation site within the noncoding region of exon 1]. Homozygous individuals for the polymorphic sequence (AA genotype) were reported to have a significant (6.9 fold) increased risk of developing asthma. This finding has not been confirmed independently. OBJECTIVE To validate the association of CC16 A38G polymorphism to asthma in a separate well-characterized population through a case-control study. METHODS We conducted an association study using a sample of 217 unrelated Northern European Caucasians. Individuals were clinically characterized by a validated respiratory questionnaire, spirometry and bronchial reactivity measurement, and genotyped for the A38G polymorphism using PCR and restriction digestion. Association analysis was performed using the nonparametric Chi-squared tests. RESULTS In the unselected population, 43.3% participants were homozygous for the CC16*G allele and 45.4% were heterozygous (AG). We observed no significant difference in the distribution of positive bronchial reactivity to methacholine (at FEV1 PC20 of |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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