Abstract 1679: Neoepitope screening in colorectal cancer patients by custom exome panel profiling somatic mutations

Autor: Raghavee Venkatramanan, Kerry Deutsch, Inah Golez, Pallavi Shroff, Spencer Seale, Brendan Ralph, Sally. Dow, Evan Anderson, Saman Tahir, Anjali Malge, Corey Braastad, Steven Anderson
Rok vydání: 2019
Předmět:
Zdroj: Cancer Research. 79:1679-1679
ISSN: 1538-7445
0008-5472
Popis: Colorectal cancer (CRC) is one of the major causes of global mortality. Recent clinical advances in T cell therapy using antibodies that block inhibitory T cell signals have been transforming cancer therapy. Studies show that somatic mutations in protein coding regions produce tumor-specific antigens, or neoantigens. CRC warrants deeper investigation into the non-synonymous exonic mutations that seem to be drivers in tumor development. Tumor mutation burden (TMB) and the abundance of neoantigens are highly correlated and it has been shown that TMB can be estimated using targeted sequencing panels. The aim of this study is to investigate 10 matched tumor/normal CRC samples using a panel targeting 422 genes covering about 1.7 Mb to screen for potential neoepitope candidates. Although sequencing and bioinformatics pipelines are developed to effectively filter neoantigens, it is still unclear which antigens elicit a T cell response. A review of the tools used in neoepitope prediction shows that it is not only a very complex task, but demands high sequencing depth. Comparing currently available tools will enable us to understand the strengths and shortcomings of each algorithm, and develop a custom pipeline framework. Note: This abstract was not presented at the meeting. Citation Format: Raghavee Venkatramanan, Kerry Deutsch, Inah Golez, Pallavi Shroff, Spencer Seale, Brendan Ralph, Sally. Dow, Evan Anderson, Saman Tahir, Anjali Malge, Corey Braastad, Steven Anderson. Neoepitope screening in colorectal cancer patients by custom exome panel profiling somatic mutations [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2019; 2019 Mar 29-Apr 3; Atlanta, GA. Philadelphia (PA): AACR; Cancer Res 2019;79(13 Suppl):Abstract nr 1679.
Databáze: OpenAIRE