Electron Microscopic Study of Erythrocyte Receptors with Labeled Antisera to Membrane Components

Autor:	C. Howe, H. Spiele, F. Minio, K. C. Hsu
Rok vydání:	1970
Předmět:	Immunology Immunology and Allergy
Zdroj:	The Journal of Immunology. 104:1406-1416
ISSN:	1550-6606 0022-1767
DOI:	10.4049/jimmunol.104.6.1406
Popis:	Hemoglobin-free membranes and erythrocytes of group O, after reaction with ferritin-labeled rabbit antibody to purified virus receptor substance, were examined by electron microscopy. Antibody was found to bind specifically to discrete sites, in thin sections of cells and ghosts, as well as in negative contrast preparations of washed membranes. The distribution of reactive sites was similar to that described by others for ferritin-labeled isoantibody. The same antibody labeled with fluorescein reacted with washed membranes to give a granular type of fluorescence. The relationship of the virus receptor substance-reactive sites to those containing blood group antigens is discussed.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::f57fc6a18102ac562719729e256525af https://doi.org/10.4049/jimmunol.104.6.1406 Zobrazit plný text záznamu