Elucidating the anti-biofilm and anti-quorum sensing potential of selenocystine against respiratory tract infections causing bacteria: in vitro and in silico studies
| Autor: | Indira K. Priyadarsini, Bharti Patel, Sirisha L. Vavilala, Subrata Mishra |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
biology Pseudomonas aeruginosa medicine.drug_class 030106 microbiology Clinical Biochemistry Antibiotics Biofilm medicine.disease_cause biology.organism_classification Biochemistry In vitro Microbiology 03 medical and health sciences chemistry.chemical_compound Quorum sensing 030104 developmental biology Extracellular polymeric substance Pyocyanin chemistry medicine Molecular Biology Bacteria |
| Zdroj: | Biological Chemistry. 402:769-783 |
| ISSN: | 1437-4315 1431-6730 |
| Popis: | Bacteria are increasingly relying on biofilms to develop resistance to antibiotics thereby resulting in their failure in treating many infections. In spite of continuous research on many synthetic and natural compounds, ideal anti-biofilm molecule is still not found thereby warranting search for new class of molecules. The current study focuses on exploring anti-biofilm potential of selenocystine against respiratory tract infection (RTI)-causing bacteria. Anti-bacterial and anti-biofilm assays demonstrated that selenocystine inhibits the growth of bacteria in their planktonic state, and formation of biofilms while eradicating preformed-biofilm effectively. Selenocystine at a MIC50 as low as 42 and 28 μg/mL effectively inhibited the growth of Klebsiella pneumonia and Pseudomonas aeruginosa. The antibacterial effect is further reconfirmed by agar cup diffusion assay and growth-kill assay. Selenocystine showed 30–60% inhibition of biofilm formation in K. pneumonia, and 44–70% in P. aeruginosa respectively. It also distorted the preformed-biofilms by degrading the eDNA component of the Extracellular Polymeric Substance matrix. Molecular docking studies of selenocystine with quorum sensing specific proteins clearly showed that through the carboxylic acid moiety it interacts and inhibits the protein function, thereby confirming its anti-biofilm potential. With further validation selenocystine can be explored as a potential candidate for the treatment of RTIs. |
| Databáze: | OpenAIRE |
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