Determination of Theophylline in Serum by Ion-Pairing Reversed-Phase High-Performance Liquid Chromatography

Autor:	Eiji Owada, Hitoshi Tada, Takahiro Kikuta, Masao Umetsu, Keiji Ito, Juichi Sato, Hisashi Motoya, Akio Wakamatsu, Hiromasa Nakata
Rok vydání:	1984
Předmět:	endocrine system Chromatography Chemistry Ion pairing Metabolite High-performance liquid chromatography chemistry.chemical_compound Phase (matter) medicine Theophylline Caffeine Sodium acetate medicine.drug Paraxanthine
Zdroj:	Japanese Journal of Hospital Pharmacy. 10:219-223
ISSN:	2185-9477 0389-9098
DOI:	10.5649/jjphcs1975.10.219
Popis:	A new specific high-performance liquid chromatographic method for the determination of theophylline (TPH) in human serum was studied. In this method, TPH in serum was extracted with chloroform-isopropanol (7: 3).; then, an aliquot of the extract was evaporated to dryness in vacuo. TPH was determined by an ion-pairing reversed-phase high-performance liquid chromatography using a Zorbax-ODS column. The mobile phase was a mixture of acetonitrilewater (5: 95 by vol) containing 10mM sodium acetate and 3mM tetrabutylammonium hydrogen sulfate (pH 6.0). Beta-hydroxyethyltheophylline was used as an internal standard.This method enables to separate TPH and paraxanthine, a metabolite of caffeine which interferes with most TPH assay procedures.
Databáze:	OpenAIRE
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