Odontogenic keratocyst expresses vascular endothelial growth factor: an immunohistochemical study
| Autor: | A. Kyroudi, G. K. Mitrou, Alexandra Sklavounou, Konstantinos I. Tosios |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Cancer Research
medicine.medical_specialty Pathology Anatomical pathology Histology Biology medicine.disease Pathology and Forensic Medicine Dentigerous cyst Vascular endothelial growth factor chemistry.chemical_compound Otorhinolaryngology Odontogenic cyst chemistry medicine Periodontics Immunohistochemistry Cyst Oral Surgery Keratocyst medicine.symptom |
| Zdroj: | Journal of Oral Pathology & Medicine. 38:470-475 |
| ISSN: | 0904-2512 |
| DOI: | 10.1111/j.1600-0714.2009.00755.x |
| Popis: | Department of Histology andEmbryology, Medical School, National and Kapodestrian University of Athens, Athens, GreeceBACKGROUND: Vascular endothelial growth factor(VEGF) expression may act as a sensitive measure of theangiogenic potential of a lesion. Furthermore, VEGF hasbeen implicated in the pathogenesis of cystic tumors andinflammatory odontogenic cysts. Thus, we studied theexpression of VEGF in the epithelium of odontogenickeratocyst (OK) in association with cell proliferation andapoptosis.METHODS: Forty-two cases of OK, 26 cases of denti-gerous cyst (DC), and 15 cases of residual cyst (RC)were retrospectively examined by immunohistochem-istry for VEGF, Ki67⁄Mib-1 and anti-caspase-3. ForVEGF and caspase-3, the intensity of immunostainingwas qualitatively assessed, while for the evaluationof Ki67 the average number of positively stained nucleiin 10 high-power microscopic fields ( ·400) was calcu-lated.RESULTS: The VEGF expression was stronger in OKwhen compared with DC ( P < 0.007). The rate of nuclearKi67 expression in OK was significantly higher than thatin DC (P < 0.001) and RC (P < 0.001). Cytoplasmic cas-pase-3 expression was statistically more intense in RCthan in OK (P = 0.001) or DC (P < 0.001). A statisticallysignificant correlation was seen in OK for Ki67 (P < 0.001)and VEGF (P = 0.023), but not for caspase-3. Multipleregression analysis revealed a linear relationship betweenVEGF and Ki67.CONCLUSIONS: The VEGF was expressed in the epi-thelium of OK, DC, and RC with a variable intensity, andin OK VEGF expression was related to Ki67. It issuggested that VEGF expression by the odontogenicepithelium is not induced solely by inflammation.J Oral Pathol Med (2009) 38: 470–475Keywords: caspases; dentigerous cyst; Ki67; odontogenic kera-tocyst; residual cyst; VEGF |
| Databáze: | OpenAIRE |
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