Incidence ofras oncogene activation in lung carcinomas in Hong Kong

Autor: Maria Pik Wong, Susan Yuk Lin Kwan, Maria Li Lung, KS Lau, Heidi H.Y. Cheung, Wah Kit Lam, Kin-hang Fu, Wing Wei Yew
Rok vydání: 1992
Předmět:
Zdroj: Cancer. 70:760-763
ISSN: 1097-0142
0008-543X
DOI: 10.1002/1097-0142(19920815)70:4<760::aid-cncr2820700407>3.0.co;2-0
Popis: Background. In Hong Kong, lung carcinomas contribute to the majority of cancer deaths among Chinese. Point mutational activation of ras oncogenes has been observed in several populations. The incidence of these mutations in Hong Kong lung carcinomas was investigated. Methods. Lung resections obtained from 52 Chinese patients whose conditions were newly diagnosed as non-small cell lung cancer, paraffin sections from 29 Chinese patients with previously diagnosed adenocarcinoma of the lung, and paraffin sections from 49 squamous cell carcinomas were examined for the presence of point mutations in Ki-ras codon 12, N-ras codon 61, and Ha-ras codon 12 oncogenes by allele-specific hybridization after specific amplification of appropriate regions of the DNA using the polymerase chain reaction. Results. Among the 130 lung carcinomas investigated, Ki-ras point mutations were detected in seven cases, of which six were adenocarcinomas and one a squamous cell carcinoma. No mutations were detected in the N-ras and Ha-ras codons. Conclusions. The incidence of Ki-ras codon 12 point mutational activation in Chinese patients with adenocarcinomas was 6 of 63 (9.5%). The incidence of Ki-ras 12 point mutational activation among men with lung adenocarcinomas in Hong Kong (6 of 32 patients, 18.8%) is significantly different from that in women in Hong Kong (0 of 31 patients, 0%). Although ras oncogenes are implicated as having a role in the development of lung adenocarcinomas, especially among smokers, it is clear from these data that they are not associated with the unusually high incidence of lung adenocarcinomas among women in Hong Kong. Cancer 1992; 70:760–763.
Databáze: OpenAIRE
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