Introduction of a plant intron into the luciferase gene ofPhotinus pyralis

Autor:	George Allen, S. Luke Mankin, William F. Thompson
Rok vydání:	1997
Předmět:	Transformation (genetics) Reporter gene biology Agrobacterium Gene expression Photinus pyralis Promoter Luciferase Plant Science Bioreporter biology.organism_classification Molecular Biology Molecular biology
Zdroj:	Plant Molecular Biology Reporter. 15:186-196
ISSN:	1572-9818 0735-9640
DOI:	10.1007/bf02812270
Popis:	We describe a new luciferase reporter gene,luc INT, for early detection of luciferase activity inAgrobacterium transformation studies, and present improved techniques for the extraction of luciferase that decrease the time needed to quantitate luciferase activity. Theluc INT reporter gene combines the PIV2 intron fromGUS INT withluc *, the modified luciferase gene.luc INTis expressed in plant cells but not inAgrobacterium, allowing earlier detection of gene expression in the presence ofAgrobacterium during transformations in tobacco leaf discs. Stable expression levels ofluc INT andluc * in tobacco suspension cultures are compared for two different promoters.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::f5515eadd5aa28132646d9767c5f9096 https://doi.org/10.1007/bf02812270 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
Nepřihlášeným uživatelům se plný text nezobrazuje	K zobrazení výsledku je třeba se přihlásit.