Popis: |
Root-knot nematodes are one of serious pathogens which cause economically losses to their host plants. Nanotechnology is a fast-growing technology in nematode and other pathogens management to avoid the hazard effects of chemical pesticides and nematicides on human health, non-target organisms and ecosystems. The effects of Ag engineered nanoparticles (AgENPs) synthesized by green method using plants with nematicidal properties or reductive potential such as Curcuma comosa (N1), Cycas circinalis (N2), Chitosan (N3) and Crotalaria juncea (N4) were tested. This study was done to investigate the genotoxic evaluation of the four AgENPs and the analysis of its effects through the assessment of pathogenicity and reproductivity of Meloidogyne incognita, plant growth parameters, silver residue, activity of antioxidative enzymes and assessment of DNA damage. The experiment was conducted using three old tomato seedlings which transplanted, treated with three concentrations of four AgENPs and infected with 1000 IJs M. incognita under screen house condition. The data demonstrated that, all nematode parameters were suppressed using the different concentrations of AgENPs. The suppressive effect was increased proportionally with the Ag concentrations. Moreover, the damage in DNA parameters was increased proportionally with concentration of AgENPs. Silver residue in tomato fruits after application is relatively very low. |