A vaccine formulated with the Chlamydia trachomatis (Ct) recombinant major outer membrane protein (rMOMP) and TLR2 and TLR9 agonists as adjuvants elicits strong protection against an intranasal challenge (166.4)
| Autor: | Chunmei Cheng, Sukumar Pal, Delia Tifrea, Luis de la Maza |
|---|---|
| Rok vydání: | 2012 |
| Předmět: | |
| Zdroj: | The Journal of Immunology. 188:166.4-166.4 |
| ISSN: | 1550-6606 0022-1767 |
| Popis: | Ct is the most common sexually transmitted bacterial pathogen in the World and there is an urgent need for a vaccine to prevent these infections. To enhance the immunogenicity of a vaccine formulated with the Ct mouse pneumonitis rMOMP, we used combinations of Pam2CSK4 (TLR2) + CpG1826 (TLR9) and Montanide ISA 720 + CpG1826 as adjuvants. Neisseria gonorrhoeae recombinant porin B was used as antigen control with the same adjuvants. Female BALB/c mice were primed twice in the nares (i.n.) or in the colon (cl.) and were boosted twice by the intramuscular plus subcutaneous (i.m.+s.c.) routes. Based on the IgG2a/IgG1 ratio in sera, mice immunized with rMOMP+Pam2CSK4+CpG1826 showed a strong Th2 response while animals vaccinated with rMOMP+Montanide+CpG1826 had a Th1 response. These mice also developed robust Ct-specific T cell proliferation and high levels of IFN-γ. Four weeks after the last immunization, the mice were challenged i.n. with 104 inclusion-forming units (IFU) of Ct. Using changes in body weight, weight of the lungs and number of IFU recovered from the lungs at 10 days post-challenge as parameters of protection, the mice immunized i.n.+i.m.+s.c. with rMOMP+Pam2CSK4+CpG1826 were better protected against the i.n. chlamydial infection than the other groups. In conclusion, a combination of Pam2CSK+CpG1826 elicits strong humoral and cellular immune responses and enhances the protective ability of a Ct-rMOMP vaccine delivered by the mucosal and systemic routes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|