| Autor: |
Pfuhlmann, Katrin, Schriever, Sonja, Legutko, Beata, Baumann, Peter, Harrison, Luke, Dhiraj Kabra, Baumgart, Emily, Tschöp, Matthias, Garcia-Caceres, Cristina, Pfluger, Paul |
| Rok vydání: |
2018 |
| DOI: |
10.6084/m9.figshare.5872971 |
| Popis: |
Effects of calcineurin inhibitor Fk506 and serum from obese mice on GFAP and vimentin levels, cell survival, and glial morphology in primary glial cultures. Representative western blot of GFAP and vimentin protein levels (a) after treatment of the primary glia cultures with FBS, obese serum, and/or Fk506 (2 or 5 μM); calcineurin inhibition was corroborated by revealing an Fk506-mediated decrease in NFATc4 protein levels; β-actin was used as housekeeping protein. Cell survival assay (b) showing cells treated with increasing concentrations of Fk506 for 48 h. Representative light microcopy images of primary astrocytes and co-isolated microglia treated with FBS or obese serum, and 2 or 5 μM Fk506 for 24 h (c), 48 h (d), and 72 h (e), respectively. Scale bar 100 μm. Representative pictures of immunocytochemical stainings (f) and quantification of fluorescence intensities to GFAP (green dye, g) in untreated, 5 μM Fk506 and 10 μM Fk506-treated primary astrocytes. Scale bar 100 μm. Means ± SEM; n = 2 biological replicates; calcineurin inhibition was confirmed once with NFATc4 (a); n = 3 biological replicates (b); representative pictures (c, d, e); quantification of GFAP staining was done in 10–11 pictures/treatment group (f, g). (PDF 817 kb) |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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