| Autor: | Kazuo Motoyoshi, Muneo Yamada, Eriko Misawa, Takuma Sakurai, Hirotoshi Hayasawa |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Pharmacology
Interleukin 2 Macrophage colony-stimulating factor Cancer Research medicine.medical_specialty medicine.medical_treatment Immunology Interleukin Biology Natural killer cell Interleukin 21 Cytokine medicine.anatomical_structure Endocrinology In vivo Internal medicine medicine Interleukin 12 Immunology and Allergy medicine.drug |
| Zdroj: | Journal of Immunotherapy. 26:21-30 |
| ISSN: | 1524-9557 |
| Popis: | The authors studied the combined effects of macrophage colony-stimulating factor (M-CSF) and interleukin (IL)-2 on the functions and antitumor activity of natural killer (NK) 1.1 + cells in vitro and in vivo. NK 1.1 + cells were isolated from the spleen of mice treated with saline or M-CSF, and their functions (proliferation, production of IFN-γ, and cytotoxicity) evaluated in vitro. Although the proliferation of and production by NK1.1 + cells was stimulated by the addition of IL-2, the cells from the M-CSF-treated mice responded better. Furthermore, the cytotoxicity against Yac-1 cells and B16 melanoma cells was stimulated by M-CSF administration and enhanced by the addition of IL-2 and IL-12. These results demonstrated that M-CSF treatment augmented the functions of NK1.1 + cells, and IL-2 and IL-12 boosted these activities in vitro. The authors then examined the effects of coadministration of M-CSF and IL-2 in vivo. The clearance of B16 cells in lung was augmented by the administration of M-CSF but not IL-2. However, M-CSF + IL-2 treatment further enhanced the clearance activity. The anti-metastatic activity was also enhanced by the M-CSF + IL-2 treatment. Furthermore, the survival of B16-bearing mice was prolonged by M-CSF + IL-2. These results suggested that administration of IL-2 boosts the functions of NK1.1 + cells, which are augmented preliminarily by the administration of M-CSF. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|