| Autor: |
Marta Ollé, Mª Jesús Cruz, Mª Dolores Untoria, Jokin Yllera, Daniel Álvarez, Xavier Muñoz |
| Rok vydání: |
2016 |
| Předmět: |
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| Zdroj: |
6.2 Occupational and Environmental Health. |
| Popis: |
Background: Clinical benefit from anti-IgE monoclonal antibody (mAb) therapy has been reported in patients with occupational asthma (OA) induced by low molecular weight (LMW) agents, which mechanisms are not well known (Lavaud F etal. Allergy 2013; 68(6):813-5). Aims and objectives: We aimed to study whether anti-IgE mAb might be suitable for treatment in a mouse model of OA due to persulfate salts. Methods: On days 1 and 8, BALB/C mice were dermally sensitized with ammonium persulfate (AP) or dimethylsulfoxide. On days 15, 18 and 21 animals were injected intraperiteonally with anti-IgE mAb or PBS 6h before an intranasal instillation of AP or saline. Airway hyperresponsiveness (AHR) was assessed using a methacholine test, and airway inflammation in bronchoalveolar lavage and total free IgE in serum samples were analyzed 24h, 48h and 96h after the last challenge. Results: Treatment with mAb completely neutralized serum IgE. Non-treated asthmatic mice showed a significant increase in AHR 24h and 48h after the last challenge and a predominantly neutrophilic inflammation along with a small influx of eosinophils and lymphocytes compared with control groups. Treatment with mAb abolished the AHR 24h and 48h after the last challenge and resulted in a significant decrease in the total number of eosinophils and neutrophils 48h and 96h after the last challenge, respectively. Histological analysis from treated mice revealed normal inflammatory patterns similar to control groups 48h after the last challenge. Conclusions: Anti-IgE treatment improved asthma features related to the AHR and airway inflammation in mice, suggesting that it can be an alternative therapy for OA induced by LMW agents. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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