| Autor: | Masanori Watanabe, Takehiro Utsunomiya, Thoru Tanaka, Toru Tanaka, Ken Sasaki |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Oxygen supply
biology Physiology Chemistry Porphyrin biosynthesis General Medicine Ala synthase Photosynthetic bacterium biology.organism_classification Photochemistry Applied Microbiology and Biotechnology Porphyrin Rhodobacter sphaeroides chemistry.chemical_compound Coproporphyrin III Biotechnology Nuclear chemistry |
| Zdroj: | World Journal of Microbiology and Biotechnology. 19:227-231 |
| ISSN: | 0959-3993 |
| DOI: | 10.1023/a:1023257228152 |
| Popis: | Porphyrin production under aerobic in the dark condition was carried out using the photosynthetic bacterium, Rhodobacter sphaeroides IFO12203 and its mutant, CR 386 which can produce 5-aminolevulinic acid (ALA) under aerobic in the dark conditions. IFO12203 produced about 1.0 mg/l of porphyrin even if 2.0 mg of ALA/l was added to the glucose–glutamate–yeast extract (GGY2) medium. However, CR 386 produced 15.0 mg/l of porphyrin after 55 h culture with the addition of 2.0 g of ALA/l and sufficient oxygen supply (dissolved oxygen, DO > 7.0 mg/l). The porphyrin produced by CR 386 consisted only of coproporphyrin III. Under conditions of strict DO control (DO = 2.0 ± 0.2 mg/l), the maximum porphyrin production attained 56.3 mg/l. Low DO (1.0 ± 0.2 mg/l) and high DO control (3.0 ± 0.2 mg/l) did not enhance porphyrin production. It is suggested that oxygen supply seems to control the step(s) of porphyrin biosynthesis of CR 386 in the stages after ALA synthase in the Shemin pathway. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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