Surface plasmon resonance measurement of pyridoxal kinase–pyridoxamine binding on self-assembled monolayer
| Autor: | Hellas C.M. Yau, Shu Yuen Wu, Ho-Pui Ho, Mengsu Yang |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Mole ratio
Stereochemistry Metals and Alloys Self-assembled monolayer Pyridoxamine binding Condensed Matter Physics Pyridoxal kinase Surfaces Coatings and Films Electronic Optical and Magnetic Materials Crystallography chemistry.chemical_compound chemistry Monolayer Materials Chemistry Gold surface Pyridoxamine Electrical and Electronic Engineering Surface plasmon resonance Instrumentation |
| Zdroj: | Sensors and Actuators B: Chemical. 85:227-231 |
| ISSN: | 0925-4005 |
| DOI: | 10.1016/s0925-4005(02)00112-0 |
| Popis: | Pyridoxamine-modified surface was prepared from self-assembly of pyridoxamine-linked 11-mercapto-undecan-1-one (PMU), which is synthesized via the chemistry of EDC/NHS activating 11-mercapto-undecanoic acid to link with pyridoxamine, on gold surface for the study of interaction between pyridoxamine and pyridoxal kinase (PK). This interaction was monitored by a low-cost red light-emitting-diode-based surface plasmon resonance technique. Addition of either 11-mercapto-undecan-1-ol (MU) or 1-dodecanethiol (DDT) to PMU for generating a mixed film can improve the performance of the monolayer of PMU for PK binding. Mixture of PMU and DDT in the mole ratio as low as 1:5 can achieve a significant enhancement (at least 89%) to the responses of PK binding. |
| Databáze: | OpenAIRE |
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