Specific Sequences Outside the E-box Are Required for Proper per Expression and Behavioral Rescue
| Autor: | Lisa C. Lyons, Steve A. Kay, Jerry H. Houl, Haiping Hao, Paul E. Hardin, Thomas K. Darlington |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
0301 basic medicine
Genetics Physiology Period (gene) Mutant E-box Biology 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Transcription (biology) Regulatory sequence Physiology (medical) Gene expression Circadian rhythm Transcription factor 030217 neurology & neurosurgery |
| Zdroj: | Journal of Biological Rhythms. 15:472-482 |
| ISSN: | 1552-4531 0748-7304 |
| DOI: | 10.1177/074873040001500604 |
| Popis: | A 69 bp circadian regulatory sequence (CRS) upstream of the per gene is sufficient to drive circadian transcription, mediate proper spatial expression, and rescue behavioral rhythmicity in per01 flies. Within the CRS, an E-box is required for transcriptional activation by two basic-helix-loop-helix (bHLH) PERARNT-SIM (PAS) transcription factors, dCLOCK (dCLK) and CYCLE (CYC). To define sequences within the CRS that are required for spatial expression, circadian expression, and behavioral rhythmicity, a series of mutants that alter blocks of 3 to 12 nucleotides across the entire CRS were used to drive lacZ or per expression in vivo. As expected, the E-box within the CRS is necessary for high-level expression and behavioral rhythmicity, but sequences outside the E-box are also required for transcriptional activation, proper spatial expression, and behavioral rhythmicity. These results indicate that the dCLK-CYC target site extends beyond the E-box and that factors other than dCLK and CYC modulate per transcription. |
| Databáze: | OpenAIRE |
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