Kaempferol inhibits the growth and metastasis of cholangiocarcinoma in vitro and in vivo
| Autor: | Baifeng Tong, Xue-wei Yang, Wu Cui, You-you Qin |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
MMP2 Cell growth Biophysics General Medicine Biology medicine.disease Biochemistry Molecular biology In vitro Metastasis 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology 0302 clinical medicine chemistry In vivo Apoptosis 030220 oncology & carcinogenesis medicine Immunohistochemistry Kaempferol |
| Zdroj: | Acta Biochimica et Biophysica Sinica. 48:238-245 |
| ISSN: | 1672-9145 |
| DOI: | 10.1093/abbs/gmv133 |
| Popis: | Kaempferol is a flavonoid that has been reported to exhibit antitumor activity in various malignant tumors. However, the role of kaempferol on cholangiocarcinoma (CCA) is largely unknown. In this article, we found that kaempferol inhibited proliferation, reduced colony formation ability, and induced apoptosis in HCCC9810 and QBC939 cells in vitro. Results from transwell assay and wound-healing assay demonstrated that kaempferol significantly suppressed the migration and invasion abilities of HCCC9810 and QBC939 cells in vitro. Kaempferol was found to decrease the expression of Bcl-2 and increase the expressions of Bax, Fas, cleaved-caspase 3, cleaved-caspase 8, cleaved-caspase 9, and cleaved-PARP. In addition, kaempferol also downregulated the levels of phosphorylated AKT, TIMP2, and MMP2. In vivo, it was found that the volume of subcutaneous xenograft (0.15 cm(3)) in the kaempferol-treated group was smaller than that (0.6 cm(3)) in the control group. Kaempferol also suppressed the number and volume of metastasis foci in the lung metastasis model, with no marked effects on body weight of mice. Immunohistochemistry assay showed that the number of Ki-67-positive cells was lower in the kaempferol-treated group than that in the control group. We further confirmed that the changes of apoptosis- and invasion-related proteins after kaempferol treatment in vivo were similar to the results in vitro. These data suggest that kaempferol may be a promising candidate agent for the treatment of CCA. |
| Databáze: | OpenAIRE |
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