1α,25-dihydroxyvitamin D3-eluting nanofibrous dressings induce endogenous antimicrobial peptide expression
| Autor: | Mark A. Carlson, Yang Zhang, Arup K. Indra, Gitali Ganguli-Indra, Richard L. Gallo, Debra A. Reilly, Jiang Jiang, Jingwei Xie, Mai N Le, Ronald R. Hollins, Hongjun Wang, Adrian F. Gombart |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Chemistry Biomedical Engineering Medicine (miscellaneous) Bioengineering Human skin Development Pharmacology Antimicrobial In vitro 03 medical and health sciences HaCaT 030104 developmental biology In vivo Nanofiber General Materials Science Antimicrobial peptide production Ex vivo |
| Zdroj: | Nanomedicine. 13:1417-1432 |
| ISSN: | 1748-6963 1743-5889 |
| DOI: | 10.2217/nnm-2018-0011 |
| Popis: | Aim: The aim of this study was to develop a nanofiber-based dressing capable of local sustained delivery of 1α,25-dihydroxyvitamin D3 (1,25(OH)2D3) and augmenting human CAMP induction. Materials & methods: Nanofibrous wound dressings containing 1,25(OH)2D3 were successfully prepared by electrospinning, which were examined in vitro, in vivo and ex vivo. Results: 1,25(OH)2D3 was successfully loaded into nanofibers with encapsulation efficiency larger than 90%. 1,25(OH)2D3 showed a sustained release from nanofibers over 4 weeks. Treatment of U937 and HaCaT cells with 1,25(OH)2D3-loaded poly(ϵ-caprolactone) nanofibers significantly induced hCAP18/LL37 expression in monocytes and keratinocytes, skin wounds of humanized transgenic mice and artificial wounds of human skin explants. Conclusion: 1,25(OH)2D3 containing nanofibrous dressings could enhance innate immunity by inducing antimicrobial peptide production. |
| Databáze: | OpenAIRE |
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