| Autor: |
Allen Smith, Carl T. Wittwer, Bonita W. Wyse, Gaurth Hansen, Won O. Song |
| Rok vydání: |
1990 |
| Předmět: |
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| Zdroj: |
Nutrition Research. 10:439-448 |
| ISSN: |
0271-5317 |
| DOI: |
10.1016/s0271-5317(05)80671-2 |
| Popis: |
We report in this study an indirect enzyme linked immunosorbent assay (ELISA) of pantothenic acid in plasma. Also reported is a comparison between the ELISA and radioimmunoassay (RIA) by measuring plasma pantothenic acid content of rats fed a pantothenic acid deficient or a surfficient diet for 1.5 and 3 wk. The ELISA is based on the competition for the antibody between immobilized pantothenate adsorbed on polystyrene microwells and free pantothenate in samples. Specific antibody was produced by injection of pantothenate-bovine serum albumin conjugate to rabbits. An optimal standard curve was produced with an antisera concentration of 1:50,000 and with anti-rabbit IgG labeled with horseradish peroxidase. The resulting A 405 was inversely correlated with free pantothenate in samples. This ELISA accurately quantitates between 2–1000 ng pantothenic acid, whereas the RIA shows linear range between 10–200 ng. The correlation coefficient (r) of 24 rat plasma pantothenic acid concentration as measured by the ELISA and RIA was 0.91. The reproducibility and recovery studies produced a 6% coefficient of variation (10 trials) and 95±6%, respectively. This new method requires no preparation for plasma. Plasma pantothenic acid concentration of rats fed a pantothenate deficient diet for 1.5 wk was significantly (p |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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