Identification of proteins overexpressed in malignant gastric tumors: Comparison of results obtained by 2DE and bioinformatic search

Autor:	George S. Krasnov, Nikolai A. Lisitsyn, Vadim L. Karpov, E. S. Grigorieva, Nadezhda V. Cherdyntseva, S. G. Afanas’ev, Yu. A. Bukurova, Tuzikov Sa, E. L. Choinzonov, S. F. Beresten
Rok vydání:	2011
Předmět:	Transcriptome Messenger RNA Two-dimensional gel electrophoresis Structural Biology Transcription (biology) Biophysics Protein Expression Analysis SOD2 Biology Proteomics Molecular biology Human genetics
Zdroj:	Molecular Biology. 45:680-685
ISSN:	1608-3245 0026-8933
DOI:	10.1134/s0026893311030058
Popis:	A comparison of protein expression profiles in intestinal and diffuse gastric tumors and normal tissues by 2D gel electrophoresis identified three proteins (SOD2, S100A6, and TXN) overexpressed in tumors. SOD2 and TXN were more frequently overexpressed in diffuse tumors than in intestinal ones. Based on the published data on comparative 2DE protein expression analysis, a control panel of eleven proteins overexpressed in gastric tumors was compiled. A bioinformatic search for the respective mRNAs in the Oncomine database (representing mRNA transcription levels in tumor vs. normal tissues) showed the agreement of proteomic and transcriptomic data for seven of 11 proteins.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::f4cd83dced53ceb0a37d3d360d8efceb https://doi.org/10.1134/s0026893311030058 Zobrazit plný text záznamu Full text from SpringerLink