| Autor: |
Kouichi Kamogawa, Remi Gloeckler, Kazuko Kodama, Denis Speck, Ikuroh Ohsawa, Tsugio Kisou, Ikuhiro Yoneda, Yves Lemoine |
| Rok vydání: |
1992 |
| Předmět: |
|
| Zdroj: |
Journal of Fermentation and Bioengineering. 73:121-124 |
| ISSN: |
0922-338X |
| Popis: |
Bacillus sphaericus was treated with N-acetylmuramidase and transformed with different bioB expression vectors by protoplast transformation, of which only three could be introduced into B. sphaericus. After cultivating the transformants for 24 h at 28°C, the culture was supplemented with pimelic acid and incubated for 48 h at 37°C. In these conditions, B. sphaericus IFO3525 transformed with a vector containing bioB under the control of the “HpaII promoter” on pUB110 accumulated 20 μg of biotin/ml in the medium. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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