A simple method to obtain endophytic microorganisms from field-collected roots

Autor:	Vanesa Analia Silvani, Mariana Pergola, Alicia Margarita Godeas, S. Fracchia, L. Fernández
Rok vydání:	2008
Předmět:	food.ingredient biology Inoculation Microorganism fungi Soil Science biology.organism_classification Microbiology Endophyte food Botany Plant species Endophytic microorganisms Agar Mycorrhiza Bacteria
Zdroj:	Soil Biology and Biochemistry. 40:1259-1263
ISSN:	0038-0717
Popis:	We describe a simple technique for obtaining endophytic microorganisms from field-collected roots. Roots of plant species were surfaced-sterilized, cut into pieces, and then each root piece was transferred to drops of Gel-Gro medium. The number of segments with bacteria and fungi was quantified and those with bacteria and dark septate endophytic (DSE) fungi were plated onto tryptic soy agar (TSA) and malt extract agar (MEA), respectively. When arbuscular mycorrhizal fungi (AMF) grew from root segments, they were inoculated into Ri T-DNA-transformed carrot root cultures. Eight different species of bacteria and five dark septate endophytes were isolated. Thirty AMF isolates were identified and continuous pure cultures established. This easy and inexpensive approach allowed us to culture various endophytic microorganisms in an in vitro system saving time and space.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::f4ba29c68f9616326799de44d714f6cf https://doi.org/10.1016/j.soilbio.2007.11.022 Zobrazit plný text záznamu Full Text from ScienceDirect