STABILIZATION OF THE ALCOHOLIC POTASSIUM HYDROXIDE IN COLORIMETRIC 17-KETOSTEROID DETERMINATIONS1

Autor:	Hildegard Wilson, Priscilla Carter
Rok vydání:	1947
Předmět:	medicine.medical_specialty Potassium hydroxide Ethanol Chromatography Reducing agent Inorganic chemistry Alcohol Ascorbic acid chemistry.chemical_compound Endocrinology chemistry Internal medicine Reagent Ketosteroid medicine Hydroxide
Zdroj:	Endocrinology. 41:417-421
ISSN:	1945-7170 0013-7227
DOI:	10.1210/endo-41-5-417
Popis:	The 2.5 normal potassium hydroxide in absolute ethanol employed in the Callow modification of the Zimmermann reaction (Callow et ah, 1938), cannot be used for more than a few days after preparation. A marked increase in the color developed in reagent blank mixtures indicates deterioration even before the solution turns yellow. Various observations suggested that these changes are primarily oxidative. It therefore appeared probable that a suitable reducing agent would stabilize the solution. Experimental findings described below reveal that incorporation of ascorbic acid in the reagent, and maintenance of an atmosphere of nitrogen, yield preparations which remain stable for at least 3 months. Preparation of 2.5 N Alcoholic Potassium Hydroxide Protected with Ascorbic Acid and Nitrogen.—One hundred ml. of absolute alcohol are measured into a glass stoppered Pyrex bottle chilled in crushed ice. Two lots of 15 mg. each of ascorbic acid are weighed.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::f4b7b9bd4aae97e887c55a4f05f7361d https://doi.org/10.1210/endo-41-5-417 Zobrazit plný text záznamu