Application of polymerized multiporous nanofiber of SnO2 for designing a bienzyme glucose biosensor based on HRP/GOx
| Autor: | Mashitah M. Yusoff, A.K.M. Kafi, Samiul Alim, Jose Rajan |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Materials science
Immobilized enzyme macromolecular substances 02 engineering and technology Biochemistry 03 medical and health sciences chemistry.chemical_compound Structural Biology Polyaniline Glucose oxidase Molecular Biology 030304 developmental biology 0303 health sciences biology technology industry and agriculture General Medicine 021001 nanoscience & nanotechnology Amperometry Electrospinning Chemical engineering chemistry Nanofiber biology.protein Cyclic voltammetry 0210 nano-technology Biosensor |
| Zdroj: | International Journal of Biological Macromolecules. 123:1028-1034 |
| ISSN: | 0141-8130 |
| DOI: | 10.1016/j.ijbiomac.2018.11.171 |
| Popis: | This work reports on a novel glucose biosensor based on co-immobilization of glucose oxidase (GOx) and horseradish peroxidase with polymerized multiporous nanofiber (MPNFs) of SnO2 onto glassy carbon electrode with chitosan. Multiporous nanofibers of SnO2 were synthesized by electrospinning method from the tin precursor which possesses high surface area good electrical conductivity, and the nanofibers were polymerized with polyaniline (PANI). GOx and HRP were then co-immobilized with the nanofibers on the surface of the glassy carbon electrode by using chitosan. The polymerized nanofibers play a significant role in facilitating the direct electron transfer between the electroactive center of the immobilized enzyme and the electrode surface. The morphology of the nanofiber and polymerized nanofiber has been evaluated by field emission scanning electron microscopy (FESEM). Cyclic Voltammetry and amperometry were employed to study and optimize the performance of the fabricated biosensor. The PANI/SnO2-NF/GOx-HRP/Ch/GC biosensor displayed a linear amperometric response towards the glucose concentration range from 5 to 100 μM with a detection limit of 1.8 μM (S/N = 3). Also, the anti-interference study and real sample analysis was investigated. Furthermore, the biosensor reported in this work exhibited excellent stability, reproducibility, and repeatability. |
| Databáze: | OpenAIRE |
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