Detection of genetic variation by MALDI-TOF mass spectrometry: rapid SNP genotyping using the GENOLINK system

Autor:	D Niemeyer, D Peters, K Fahr, Thomas J. Wenzel, M Kostrzewa, E Petkovski, T Elssner
Rok vydání:	2004
Předmět:	Mutation rate Matrix-assisted laser desorption/ionization Polymorphism (computer science) Genetic variation Single-nucleotide polymorphism Human genome General Medicine Computational biology Biology Mass spectrometry Molecular biology SNP genotyping
Zdroj:	International Congress Series. 1261:9-11
ISSN:	0531-5131
DOI:	10.1016/s0531-5131(03)01836-3
Popis:	Single nucleotide polymorphisms (SNPs) are the most common form of genetic variation in the human genome. Because of their low mutation rate, SNPs have the potential to be as discriminating as loci of higher polymorphism, e.g. for forensic approaches such as paternity tests or human identification in general. To balance the lack of polymorphic information the number of markers which are tested has to be increased. Here, we introduce a new automated platform for SNP genotyping by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF MS), easy to use as well for higher DNA sample throughput as also for the analysis of an increased number of SNPs.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::f4940006fa773585c4cf17b77c23523a https://doi.org/10.1016/s0531-5131(03)01836-3 Zobrazit plný text záznamu Full Text from ScienceDirect