Pairing of segmentation clock genes drives robust pattern formation
| Autor: | Ahmet Ay, Kemal Keseroğlu, Oriana Q. H. Zinani, Ertuğrul M. Özbudak |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0303 health sciences
Multidisciplinary Robustness (evolution) Computational biology Biology biology.organism_classification Phenotype 03 medical and health sciences 0302 clinical medicine Pairing embryonic structures Gene expression Segmentation Cellular noise Zebrafish Gene 030217 neurology & neurosurgery 030304 developmental biology |
| Zdroj: | Nature. 589:431-436 |
| ISSN: | 1476-4687 0028-0836 |
| Popis: | Gene expression is an inherently stochastic process1,2; however, organismal development and homeostasis require cells to coordinate the spatiotemporal expression of large sets of genes. In metazoans, pairs of co-expressed genes often reside in the same chromosomal neighbourhood, with gene pairs representing 10 to 50% of all genes, depending on the species3–6. Because shared upstream regulators can ensure correlated gene expression, the selective advantage of maintaining adjacent gene pairs remains unknown6. Here, using two linked zebrafish segmentation clock genes, her1 and her7, and combining single-cell transcript counting, genetic engineering, real-time imaging and computational modelling, we show that gene pairing boosts correlated transcription and provides phenotypic robustness for the formation of developmental patterns. Our results demonstrate that the prevention of gene pairing disrupts oscillations and segmentation, and the linkage of her1 and her7 is essential for the development of the body axis in zebrafish embryos. We predict that gene pairing may be similarly advantageous in other organisms, and our findings could lead to the engineering of precise synthetic clocks in embryos and organoids. The pairing of genes is essential for robust expression of segmentation clock genes during development in zebrafish embryos. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink