| Autor: |
Alexandria B. Boehm, Linlin Li, Lorelay M. Mendoza, Nasa Sinnott-Armstrong, Kevan M. Yamahara, Stephanie K. Loeb, Katherine E. Graham, Laura Roldan-Hernandez, David Catoe, Lauren M. Sassoubre, Marlene K. Wolfe, Krista R. Wigginton, Sooyeol Kim |
| Rok vydání: |
2020 |
| Předmět: |
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| DOI: |
10.1101/2020.09.14.20194472 |
| Popis: |
Wastewater-based epidemiology (WBE) may be useful for informing public health response to viral diseases like COVID-19 caused by SARS-CoV-2. We quantified SARS-CoV-2 RNA in wastewater influent and primary settled solids in two wastewater treatment plants to inform the pre-analytical and analytical approaches, and to assess whether influent or solids harbored more viral targets. The primary settled solids samples resulted in higher SARS-CoV-2 detection frequencies than the corresponding influent samples. Likewise, SARS-CoV-2 RNA was more readily detected in solids using one-step digital droplet (dd)RT-PCR than with two-step RT-QPCR and two-step ddRT-PCR, likely owing to reduced inhibition with the one-step ddRT-PCR assay. We subsequently analyzed a longitudinal time series of 89 settled solids samples from a single plant for SARS-CoV-2 RNA as well as coronavirus recovery (bovine coronavirus) and fecal strength (pepper mild mottle virus, PMMoV) controls. SARS-CoV-2 RNA targets N1 and N2 concentrations correlate positively and significantly with COVID-19 clinical confirmed case counts in the sewershed. Together, the results demonstrate that measuring SARS-CoV-2 RNA concentrations in settled solids may be a more sensitive approach than measuring SARs-CoV-2 in influent.Abstract Figure |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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