Recombinase Polymerase Amplification Combined with Unmodified Gold Nanoparticles for Salmonella Detection in Milk
| Autor: | Zhi-guang Chen, Luo Huan, Hai-xia Zhong, Jun-rong Huang, Ren-yu Zhang |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Salmonella
Food spoilage Recombinase Polymerase Amplification medicine.disease_cause 01 natural sciences Applied Microbiology and Biotechnology Analytical Chemistry chemistry.chemical_compound 0404 agricultural biotechnology medicine National standard Safety Risk Reliability and Quality Detection limit Chromatography biology Chemistry 010401 analytical chemistry 04 agricultural and veterinary sciences biology.organism_classification 040401 food science 0104 chemical sciences Colloidal gold Safety Research Bacteria DNA Food Science |
| Zdroj: | Food Analytical Methods. 12:190-197 |
| ISSN: | 1936-976X 1936-9751 |
| Popis: | Salmonella is one of the most common pathogenic bacterium, which causes food spoilage and human diseases. The national standard detection method (GB4789.4) and other fast detection methods for Salmonella cannot satisfy the requirements in the present climate because of their inefficiency, low accuracy, high equipment requirements, or high cost. In this study, we combine isothermal recombinase polymerase amplification (RPA) with unmodified gold nanoparticles (AuNPs) to detect Salmonella in milk. A rapid, effective, visualized, and low-cost RPA-AuNP assay was developed, with a detection limit of 50 CFU for milk samples after enrichment for 6 h or 1 pg for DNA within 15 min at 37 °C using simple water bath equipment. Furthermore, it exhibited no cross-reactions with other pathogens by using highly specific region of invA of Salmonella as the target detection gene. And it may apply to the local food inspection and detection center whose equipment conditions are poor. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink