| Autor: |
Cordula Grüttner, Annika Kasten, Birte J. Siegmund, Jens-Peter Kühn, Bernhard Frerich |
| Rok vydání: |
2015 |
| Předmět: |
|
| Zdroj: |
Journal of Magnetism and Magnetic Materials. 380:34-38 |
| ISSN: |
0304-8853 |
| DOI: |
10.1016/j.jmmm.2014.08.044 |
| Popis: |
Magnetic resonance imaging (MRI) is to be considered as an emerging detection technique for cell tracking experiments to evaluate the fate of transplanted progenitor cells and develop successful cell therapies for tissue engineering. Adipose tissue engineering using adipose tissue-derived progenitor cells has been advocated for the cure of soft tissue defects or for persistent soft tissue augmentation. Adipose tissue-derived progenitor cells were differentiated into the adipogenic lineage and labeled with two different types of magnetic iron oxide nanoparticles in varying concentrations which resulted in a concentration-dependent reduction of gene expression of adipogenic differentiation markers, adiponectin and fatty acid-binding protein 4 (FABP4), whereas the metabolic activity was not altered. As a result, only low nanoparticle concentrations for labeling were used for in vivo experiments. Cells were seeded onto collagen scaffolds and subcutaneously implanted into severe combined immunodeficient (SCID) mice. At 24 h as well as 28 days after implantation, MRI analyses were performed visualizing nanoparticle-labeled cells using T2-weighted sequences. The quantification of absolute volume of the scaffolds revealed a decrease of volume over time in all experimental groups. The distribution of nanoparticle-labeled cells within the scaffolds varied likewise over time. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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