Comparative Analysis of the Reactivity of Diazirine-Based Photoaffinity Probes toward a Carbohydrate-Binding Protein

Autor:	Tomoki Yasui, Sakae Mizuno, Kaori Sakurai
Rok vydání:	2015
Předmět:	chemistry.chemical_classification Lysis Photoaffinity labeling Stereochemistry Binding protein Organic Chemistry Carbohydrate-binding protein Alkyne chemistry.chemical_compound chemistry Diazirine Organic chemistry Reactivity (chemistry) Alkyl
Zdroj:	Asian Journal of Organic Chemistry. 4:724-728
ISSN:	2193-5807
DOI:	10.1002/ajoc.201500116
Popis:	A set of lactose-based photoaffinity probes bearing either alkyl diazirine or trifluoromethylphenyl diazirine (TPD) groups were designed and synthesized to directly compare their efficiency in photocrosslinking a carbohydrate-binding protein. The crosslinking efficiency of the TPD probes was higher than the alkyl diazirine probes when reacted with a single binding protein. However, the alkyl diazirine probe with a small alkyne tag achieved more selective photoaffinity labeling of a binding protein in cell lysate than the corresponding TPD probe.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::f47397050201e8ca8a2856c686f1124d https://doi.org/10.1002/ajoc.201500116 Zobrazit plný text záznamu Plný text