Effect of surface structure on cell growth prepared by the terminal immobilization method
| Autor: | Masashi Kunitake, Chyuichi Hirayama, Masayo Sakata, Hiroshi Mizokami, Hirotaka Ihara, Toshihiko Sakurai, Shinichi Furukawa |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
chemistry.chemical_classification
Polymers and Plastics Cell growth Chemical structure Cell Substrate (chemistry) General Chemistry Surfaces Coatings and Films Amino acid chemistry.chemical_compound medicine.anatomical_structure chemistry Cell culture Polymer chemistry Materials Chemistry Biophysics medicine Moiety Phenyl group |
| Zdroj: | Journal of Applied Polymer Science. 91:3001-3008 |
| ISSN: | 1097-4628 0021-8995 |
| DOI: | 10.1002/app.13501 |
| Popis: | Substrate effects of surface morphology and chemical structure for cell cultures prepared by molecular terminal immobilization method were studied. When we focused attention on a phenyl group as a functional moiety, the cell growth on the surface prepared by the immobilization method showed a better proliferation rate than that of a substrate prepared by the casting method. Further, from the results of mouse fibroblast L929 cell (L-cell) growth on poly(amino acid)-immobilized surfaces in Dulbecco's minimal essential medium containing 10% FBS, it was indicated that the amino group was more effective than the phenyl group, and that a slight difference of chemical structure had a substantial influence on cell growth. In addition, mouse bone marrow–derived Sp2/0-Ag14 cell (Sp2/0 cell) culture in ASF-104 serum-free medium, poly(amino acid)-immobilized substrates showed an almost equal proliferation rate to that in a serum-containing medium. These results showed that effective cell growth can occur on immobilized surfaces, and that detection of a weak interaction depends on the functional groups. © 2004 Wiley Periodicals, Inc. J Appl Polym Sci 91: 3001–3008, 2004 |
| Databáze: | OpenAIRE |
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