High Performance Liquid Chromatographic Determination of Pseudoephedrine in Plasma in the Presence of Guaifenesin
| Autor: | Vaishali Pade, Salomon A Stavchansky |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Guaifenesin
Detection limit Chromatography Chemistry Coefficient of variation Biochemistry (medical) Clinical Biochemistry Pseudoephedrine Biochemistry High-performance liquid chromatography Analytical Chemistry Nasal decongestant Electrochemistry medicine Sample preparation Solid phase extraction Spectroscopy medicine.drug |
| Zdroj: | Analytical Letters. 26:867-873 |
| ISSN: | 1532-236X 0003-2719 |
| Popis: | A high performance liquid chromatography method with uv detection was developed for the quantitation of pseudoephedrine in human plasma in the presence of guaifenesin. The method involved a sample preparation technique based on solid phase extraction, followed by evaporation, reconstitution in water and injection into the HPLC system. The HPLC system consisted of a C8 column and a 70:30 V/V 0.03M sodium heptane sulfonate : acetonitrile as the mobile phase, ultraviolet detector at 220 nm and (±)α-(methylaminomethyl) benzyl alcohol (MAMBA) as the internal standard. The concentration-response relationship for pseudoephedrine was found to be linear over a concentration range of 25 ng/ml to 450 ng/ml, with a lower limit of detection of 20 ng/ml and a lower limit of quantitation of 25 ng/ml. The coefficient of variation for inter-day ranged from 5.7% to 10.5% with a mean of 8.3% over the concentration range of 70–450 ng/ml. The coefficient of variation for intra-day ranged from 1.1% to 5.1% with a mean... |
| Databáze: | OpenAIRE |
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