Untersuchungen zur Biokompatibilität eines neuen ionomeren Knochenzementes in der Endoprothetik

Autor:	D. B. Jones, Ulrich Meyer, Szulczewski Dh, Wuisman P, K. Moller
Rok vydání:	2008
Předmět:	musculoskeletal diseases Cement Biocompatibility business.industry Chemistry Dentistry Biomaterial Osteoblast Bone cement Molecular biology Staining medicine.anatomical_structure Cell culture medicine Orthopedics and Sports Medicine Surgery business Immunostaining
Zdroj:	Zeitschrift für Orthopädie und ihre Grenzgebiete. 134:117-124
ISSN:	1438-941X 0044-3220
DOI:	10.1055/s-2008-1039782
Popis:	Periosteal derived bovine osteoblasts and osteoclasts migrated in culture onto an ionomeric cement, currently evaluated as a cement for orthopaedic implants. Cell cultures were maintained for 4 weeks and used to study the in-vitro behaviour of cells on the material surface. Osteoblasts and osteoclasts colonized the substrate in monolayers and exhibited their phenotypic morphology. Differentiation into both cell lines were demonstrated by immunostaining. Staining for aluminium in cells growing on the bone cement showed an uptake and storage of aluminium in the cells. EDAX-microanalysis revealed high concentrations of Al and Si in the periosteal tissue. Despite uptake of Al in the cells, signs of toxicity were not apparent in the cell culture system.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::f44222e21e6b675132d6e725eb82bc49 https://doi.org/10.1055/s-2008-1039782 Zobrazit plný text záznamu