Metabolism of Isolated Hepatopancreas Cells from the Blue Crab (Callinectes sapidus) under Simulated Postexercise and Hypoxic Conditions
| Autor: | F. H. Lallier, P. J. Walsh |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Callinectes
Physiology Decapoda Oxygene Environmental factor chemistry.chemical_element Metabolism Biology biology.organism_classification medicine.disease_cause Oxygen Endocrinology Biochemistry chemistry Physiology (medical) medicine Animal Science and Zoology Hepatopancreas Respiratory system computer Nuclear chemistry computer.programming_language |
| Zdroj: | Physiological Zoology. 65:712-723 |
| ISSN: | 0031-935X |
| DOI: | 10.1086/physzool.65.4.30158535 |
| Popis: | The metabolic fate of lactate (and incidentally alanine) and the occurrence and importance of gluconeogenic processes in the hepatopancreas in Crustacea are still controversial. The use of isolated cells seemed appropriate to address this problem more directly. Cells isolated from the hepatopancreas of blue crabs, Callinectes sapidus, were suspended in solutions reproducing as closely as possible actual in vivo acid-base and respiratory conditions during four different situations: control (C, pH = 7.6, CO₂ = 0.25%, O₂ = 21%, [L-lac] = 0.5 mM, [Ala] = 0.5 mM), hypoxia (H, pH = 7.9, CO₂ = 0.1%, O₂= 2%, [L-lac] = 0.5 mM, [Ala] = 0.5 mM), postexercise (E, pH = 7.2, CO₂ = 1%, O₂= 21%, [L-lac] = 15 mM, [Ala] = 5 mM), and recovery (R, pH = 7.6, CO₂ = 0.25%, O₂= 21%, [L-lac] = 5 mM, [Ala] = 5 mM). We measured the production of CO₂ and glucose from $^{14}C-lactate$ and $^{14}C-alanine$ and the production of CO₂ from $^{14}C-glucose$. Carbon dioxide production from lactate was low in C (0.11 μmol · g⁻¹ · h⁻¹) and H... |
| Databáze: | OpenAIRE |
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