| Autor: |
Tavia Rucker, Shih-hua Tan, Georgios Vidalakis, Deborah Pagliaccia, Fatima Osman, Jinbo Wang, Irene Lavagi-Craddock, Sohrab Bodaghi, Tyler Dang |
| Rok vydání: |
2021 |
| Předmět: |
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| Zdroj: |
Methods in Molecular Biology ISBN: 9781071614631 |
| Popis: |
High-throughput nucleic acid extraction is critical for the implementation of modern viroid detection assays. Successful large-scale nursery, field surveys, and other regulatory, quarantine, or research diagnostic programs are increasingly dependent on high-throughput tissue pulverization and nucleic acid extraction protocols. Magnetic bead-based approaches using semi-automated robotic equipment allow high-throughput extraction and purification of high-quality uniform total nucleic acids for each individual sample. Here, we describe a streamlined and optimized protocol for citrus tissue processing and RNA extraction that can be used for downstream applications such as viroid detection by reverse transcription-quantitative polymerase chain reaction. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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